ABSTRACT
The last century has witnessed the introduction, establishment and expansion of mosquito-borne diseases into diverse new geographic ranges. Malaria is transmitted by female Anopheles mosquitoes. Despite making great strides over the past few decades in reducing the burden of malaria, transmission is now on the rise again, in part owing to the emergence of mosquito resistance to insecticides, antimalarial drug resistance and, more recently, the challenges of the COVID-19 pandemic, which resulted in the reduced implementation efficiency of various control programs. The utility of genetically engineered gene drive mosquitoes as tools to decrease the burden of malaria by controlling the disease-transmitting mosquitoes is being evaluated. To date, there has been remarkable progress in the development of CRISPR/Cas9-based homing endonuclease designs in malaria mosquitoes due to successful proof-of-principle and multigenerational experiments. In this review, we examine the lessons learnt from the development of current CRISPR/Cas9-based homing endonuclease gene drives, providing a framework for the development of gene drive systems for the targeted control of wild malaria-transmitting mosquito populations that overcome challenges such as with evolving drive-resistance. We also discuss the additional substantial works required to progress the development of gene drive systems from scientific discovery to further study and subsequent field application in endemic settings.
ABSTRACT
Events, such as the COVID-19 pandemic, that rapidly impact global communication and travel have significant consequences for the tourism industry, which is one of the pillars of global development. We assess the impacts of the COVID-19 crisis on the Colca y Volcanes de Andagua UNESCO Global Geopark in Peru. The Colca y Volcanes de Andagua Geopark was established immediately prior to the pandemic in October 2019. The instability of the government in Peru during the pandemic and the difficult living conditions in the high Andes, such as the lack of drinking water, cleaning agents, medical care, and the high levels of poverty, particularly in the geopark region, has contributed to the significantly high COVID-19 infection rates. In addition, detrimental impacts faced by the local community are a direct result of a reduction in travellers to the area due to legislative restrictions, which have had negative consequences on the local tourism industry. There is an urgent need for the recovery of the local tourism industry to prevent the permanent closure of tourism facilities and to minimise poverty rates.